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KMID : 0363219930310060920
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Korean Journal of Dermatology
1993 Volume.31 No. 6 p.920 ~ p.930
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The Effect of Free Oxygen Radical Scavengers and Interleukin-2 on the UVB-induced Suppression of Human Natural Killer activity in Vitro
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Kim Kwang
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Abstract
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Background:
@EN Previous studies have shown that in vitro exposure to ultraviolet B(UVB) radiation resulted in a dose-dependent inhibition of natural killer activity(NK activity) of normal human peripheral blood mononuclear cells(PBMC), and that in vivo
exposure to
sunlight also induced NK activity suppression. The precise mechanism of the UV-regulation on the natural killer system(NK system) is not established.
@ES Objective & Methods:
@EN The purpose of this study is to examine whether the addition ofinterleukin-2(IL-2) and/or free oxygen radical scavengers, superoxide dismutase(SOD) or sodium azide(SA), is effective in reducing the UVB-induced suppression of NK activity of
PBMC..
@ES Results: The results are as follows
@EN 1. The suppressive effect of UVB radiation on NK activity could successfully be prevented in the presence of SOD(100 and 1,000U/ml) during the radiation. 2. SA(10E-4 and 10E-2M/ml) did not prevent the suppression of NK activity. 3.
IL-2(100U/ml)
markedly enhanced the NK activity of nonirradiated PBMC, but had no effect on irradiated PBMC. 4. Combination treatment with both IL-2 and free radical scavengers on UVB-irradiated PBC resulted in noadditive or synergistic effect on the
preventioin
of
the suppression of NK activity compared with a single treatment with either IL-2 or free radical scavengers.
@ES Conclusion:
@EN In the pressent study, we found that SOD provided a protective effect on NK activity during the UVB radiation and we suggest that superoxide anion(O2-) might play a major role in the UV-regulatory mechaisms of the NK system (Kor J Dermatol
1993;31(6): 920-930)
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KEYWORD
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